EPSCoR in Idaho

 

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2006 IBEST Bioinformatics Workshop

August 14-16, 2006

University of Idaho

Janssen Engineering Blg. (JEB) 211a

Moscow, Idaho

Workshop Agenda

Time

Topic

 

Monday, August 14 -  Celeste Brown

8:00 am

Workshop Introduction

 
8:05 am

Introduction to IBEST Computers

IBEST Network

 

 8:10 am

Unix

 

9:05 am

Word-processing in UNIX

  • PICO

 
9:20 am

Logging on and getting your data to the computers

  • SSH-Secure shell client on Windows
  • ssh and scp on Mac or Unix
 
9:30 am

Running programs on Solaris, Linux, OSX-Unix

Introduction to programs on the IBEST system

  • EMBOSS
  • Blastall
  • Clustalx/Clustalw
  • Phylip/PAUP*

 
11:00 am Basic Shell scripting  
Noon Lunch  
1:00 pm

IBEST Cluster Computers: oceanus and phlegathon

  • Sun Grid Engine
  • qsub
  • qstat

 
2:00 pm

Web-based tools

  • Nucleic Acids Research Database issue
  • BioToolBox
  • Biology Workbench
  • PISE

 
3:00 pm

An introduction to Vector NTI

 
 

Tuesday, August 15th - Matt Settles

8:00 am

Workshop Introduction

 
8:05 am

Introduction to Microarray Analysis

  • The biology of microarrays

  • Two Channel vs Single Channel microarrays

 

 
8:30 am

An Introduction to R and Bioconductor

  • Websites
  • Installing R and add-on packages
    • Tutorials 1, 2         

 
9:30 am

Two Channel  - cDNA arrays

  • Quality Control

  • Preprocessing
  • Normalization
 
10:30 am

Single Channel - Affymetrix

  • Quality Control
  • Preprocessing
  • Normalization
 
11:30 am

Differential Expression

  • Limma
  • multtest

 
Noon

LUNCH sponsored by Insightful

Dr. David Henderson 

 
1:00 pm

DE continued and Annotating Lists of Interesting Genes

  • Chip Annotation
  • Meta-data

 
2:00 pm

Advanced Topics

  • Clustering and Heatmaps
  • Gene Ontology Analysis
  • Machine Learning / Classification

 
3:30 pm

Additional Software

  • BRB array tools
  • GeneSpring GX
  • ExpressionNTI

 
     

Wednesday, August 16th - Celeste Brown and Matt Settles

8:00 am

Celeste Brown and Matt Settles will be available to aid those who attended Monday and Tuesday sessions in analysis of their own data.

 
Noon

Lunch sponsored by Affymetrix

Mark Daly